The effect of Anthocleista djalonensis leaves on the zone of inhibition of microorganism isolated from fruits (Watermelon, pineapple, apple and orange) juice stored at 30+10C for 20days were investigated using agar diffusion and dilution methods. The result obtained reveled that the Zone of inhibition Staphylococcus aureus was 18mm (juice isoaltes), clinical 17mm while typed culture was 13mm. Bacillus cereus was 7mm (clinical isolate), while juice isolate was 10mm. The minimum inhibitory concentration (MIC) ranged from 50 – 250mg/ml in all the isolates while the minimum bacteriocidal concentration (MBC) ranged from 100 – 300mg/ml. The Bacillus subtilis and Bacillus cereus, were more susceptible to Anthocleista djalonensis  than the rest strains.