A total number of 60 samples of water leaf were collected from different areas in Enugu Metropolis, these samples were inoculated and examined to detect the presence of sorbitol negative Escherichia coli 0157:H7. About 35(17.5%) samples out of the 50 samples inoculated were found to be sorbitol negative E-coli. From the result E-coli 0157:H7 is the predominant serotype accounting for 90% of all VTEC isolates.

TABLE OF CONTENTS

Title Page

Certification Page

Dedication

Acknowledgement

Abstract

Table of Contents

CHAPTER ONE

1.0 Introduction

1.1 AIM

CHAPTER TWO

2.0 Literature Review

2.1 Historical Development of Escherichia Coli

2.2 Natural Habitat

2.3 Epidemology of E-coli 0157:H7

2.4 Mode of Transmission

2.5 Pathophysiology

2.6 Pathogenicity 

2.7 Risk Factors for Transmission

2.8 Laboratory Diagnosis

2.9 Signs and Symptoms

2.10 Prevention and Control

2.11 Treatment

CHAPTER THREE

3.0 Materials and Methods

3.1 Materials Used

3.2 Collection of Sample

3.3 Media Preparation

3.4 Inoculation of samples into sorbitol MacConkey

3.5 Method of Confirming E-coli

3.6 Isolation of sorbitol negative Escherichia coli

3.7 Identification of E-coli 0157:H7 using H7 serology and toxin testing

3.8 Identification of E-coli using MUG

3.9 Identification of E-coli 0157:H7 using BCIG

3.10 Identification of E-coli using slide agglutination test

CHAPTER FOUR

4.0 Result

CHAPTER FIVE

5.0 Discussion

5.1 Conclusion

5.2 Recommendation

REFERENCES