ABSTRACT

In the for anti microbial effect the back peel of Persea americana (AVACADO) was investigated for activities . the study was to evaluate  the antimicrobial efficacy of  the crude ethanolic and aqueous extract of the peel of  Persea americana against  selected clinical isolates. The agar well diffusion method was used in study also phytochemical screening was conducted on the plant which revealed the presences of saponin, carbohydrates, tannins the  present invention relates to extracts from Persea americana enriched in bioactive compounds which can be used as antimicrobial, antibacterial or spore germinating inhibiting agents the process for obtaining the extract is to isolate molecules and method for using the extracts enriched in bioactive compounds for providing antimicrobial inhibiting effect.

CHAPTER ONE

Introduction - - - - - - - - - - 1

1.1 Background to the study - - - - - - - 1

CHAPTER TWO

Literature review - - - - - - - - - 3

2.1 Avocado - - - - - - - - - 3

2.2 Historical origin of the plant - - - - - - - 3

2.3 Varieties of avocado - - - - - - - - 3

2.4 Scientific classification of avocado pear - - - - - 6

2.5 Cultivation - - - - - - - - - 7

2.6 Avocado propagation - - - - - - - - 8

2.7 Pest and diseases affecting avocado - - - - - - 8

2.8 Harvest and post harvest of avocado - - - - - - 9

2.9 Health benefit of avocado - - - - - - - 9

2.10 Chemical composition of avocado - - - - - - 11

2.10.1 Fatty acid composition - - - - - - - 11

2.10.2 Phytochemicals composition - - - - - - - 11

2.10.3 Pharmacological activities of avocado - - - - - 12

2.11 Allergies - - - - - - - - - 13

CHAPTER THREE

3.0 Materials and method - - - - - - - - 14

3.1 Materials - - - - - - - - - - 14

3.1.1 Chemicals and reagents - - - - - - - - 14

3.2 Equipments and glass wares - - - - - - - 14

3.3  Sample collection - - - - - - - - - 15

3.4 Plants extract preparation - - - - - - - - 16

3.5 Test organism used for the analysis - - - - - - 16

3.6 Antimicrobial susceptibility test - - - - - - - 16

CHAPTER FOUR

4.0 Results - - - - - - - - - - 18

CHAPTER FIVE

5.1 Discussion - - - - - - - - - - 19

Conclusion - - - - - - - - - - 21

Recommendation - - - - - - - - - 21

References - - - - - - - - - - 22