Research and reviews are constantly being carried out in order to discover newer, and abundant plant sources of bioactive components which exhibit strong pharmacognostic and biochemical effects. The whole plant of Diodia sarmentosa has a folkloric reputation as an anti-ulcer agent. It is claimed to be useful in herbal medicine for the treatment of bacterial and fungal infections and some other ailments. The qualitative and quantitative analysis of some phytochemicals in Diodia sarmentosa (tropical buttonweed) leaf were determined using two forms of the plant - the aqueous extract and pulverised samples of the leaf  - so as to determine and/or confirm the presence of some phytochemicals with significant biological activity.