ABSTRACT Objective: There is currently little information on the acceptability of male circumcision In Uganda, This study investigated the acceptability of male circumcision among Mothers in Kiryandongo district with male children. Design: A cross-sectional survey was conducted among a convenience sample of 100 women attending a reproductive health clinic at the OPD waiting area in Kiryandongo district hospital in Uganda between February and April 2018. Results: Of the 100 invited eligible participants, the majority of women were Christians (78%), (18%) were Muslims, and (4%) were Pentecostal. About 60% of respondents had at least attained primary education (28%) had secondary education 9% had tertiary education and only 3% had no education at all .After women were informed about the risks and benefits of male circumcision, majority of women with uncircumcised children 81% said they would definitely circumcise their children if the procedure were offered in a safe hospital setting, free of charge and a smaller number (7%) said they would probably consider the procedure. Only (1%) said that they would definitely probably not consider male circumcision, and (9%) were unsure. Conclusion: Since male circumcision has been found to decrease risk of HIV infection. Among men, it is important to determine its acceptability as a potential HIV prevention Strategy at even an early stage in lie in Uganda. This study found male circumcision to be highly acceptable among a broad range of mothers with male children in Kiryandongo district Uganda. Further studies of acceptability among fathers and other populations are warranted.