ABSTRACT This was a prospective cross-sectional survey study conducted in the Radio diagnostic Department of National Hospital Abuja from June 2013 to January 2014 on 210 cases (111 males and 99 females). The specific objectives were to determine the: (i) biometric values of corpus callosum in an adult Nigerian population, (ii) differences in corpus callosal biometry among different sexes and (iii) relationship between age, body mass index and corpus callosal biometry.The following findings were made from the study: The mean corpus callosal biometry for the total population were: LC; 7.76±0.57cm, HC; 2.45±0.28cm, WG; 1.19±0.19cm, WS; 1.17±1.20cm, WC; 0.62±0.01cm, WCA; 0.58±0.01cm, WCP; 0.49±0.01cm, LR; 1.78±0.25cm. The mean corpus callosal biometry for the males were: LC; 7.82±0.45cm, HC; 2.46±0.29cm, WG; 1.20±0.19cm, WS; 1.17±1.21cm, WC; 0.62±0.10cm, WCA; 0.49±0.09cm, WCP; 0.49±0.07cm, LR; 1.78±0.25cm. The mean corpus callosal biometry for the females were: LC; 7.69±0.91cm, HC; 2.43±0.28cm, WG; 1.17±0.18cm, WS; 1.18±1.20cm, WC; 0.64±0.01cm, WCA; 0.59±0.08cm, WCP; 0.49±0.08cm, LR; 1.77±0.22cm. There was no significant difference (P >0.05) in the mean values of corpus callosal biometry in males when compared to the females. There was no significant relationship (P > 0.05) between corpus callosal biometry with age and BMI. The normal values from this work can give a clue in making diagnosis of some brain disease processes. Hence, the effect of brain diseases on the corpus callosum can further be researched upon among the Nigerian population.