TABLE OF CONTENTS
Contents pages
Title page i
Certification ii
Dedication iii
Acknowledgements iv
Table of Contents v
Abstract vi
CHAPTER ONE
1.0 Introduction 1
1.1 Background Study 1
1.2 Statement of the problem 2
1.3 Justification 3
1.4 Aim and Objectives of Study 4
CHAPTER TWO
2.0 Literature review 6
2.1 Definition and history of Malaria 6
2.1.2 Etiologic and vectors of malaria 7
2.1.3 Epidermiology of malaria 8
2.1.4 Life cycle of malaria parasite 10-13
2.1.5Molecular cell biology and pathogenesis 13
2.1.6Diagnosis of malaria 14
2.1.7 Management of malaria 18
2.1.7.1Conventional therapeutic agents 18
2.1.7.2 Drug in pipeline 19
2.2 Traditional medicine 21
2.2.1 Control measures 22
2.3 Malaria vaccine 24
2.4 The experimental plant 25
2.4.1Moringa Oleifera 26
2.4.2 Social Economic importance of morning oleifera 29
2.4.3 Ecology and Cultivation 29
CHAPTER THREE
3.0 Collection of plant 42
3 .1 Control drugs 42
3.2 Experimental animal 43
3.3 Materials and reagent 43
3.4 Extraction from the plant seed 43
3.5 Gas chromatography mass spectrometry 44
3.6. Experimental Design 50
3.7 Collection and inoculation of the parasite 50
3.8 Statistical Analysis 52
3.9 Presentation and statistical analysis of Data 52
CHAPTER FOUR
4.0 Result 53
4.1 Parasite density at different concentration of the extract of Maringa oliefera seed 55
4.2 Percentage difference in parasitaemia inhibition at different concentration among
seed 58
CHAPTER FIVE
5.0 Discussion 60
5.1 Conclusion 61
5.2 Recommendation 62
ABSTRACT
Malaria is an increasing worldwide threat, with more than three hundred million infections and one million deaths every year. Due to the emergence of antimalarial drug resistance, the continuous search for antimalarial agents. This study was conducted to determine the antimalarial efficacy of Moringa oleifera Seed extract in Swiss albino mice infected with Plasmodium berghei .After extraction, phytochemical screening and gas chromatographic mass spectrometry (GC-MS) screening of the extract, the mice were grouped into six groups, five per group. Designated as 40% treated with 40mg/kg of the Maringa oliefera seed extract, 60% treated with 60mg/kg, 80% treated with 80mg/kg,100% treated with 100mg/kg and positive control treated with distilled water while negative control was given choloroquone. For the period of 3 days at 12 hours interval. Parasite density was determine by preparing of thick and thin blood film, stain with Giemsa stain and view under microscope to determine the antiplamodial activity of the extract
