ABSTRACT

Plants have long been a source of nutrition and therapy to man. The medicinal value of these plants lies in some chemical substances that produce a definite physiological action in the human body. This study evaluated the oxytocic potential of methanol extract of Sida acuta on female albino rats in vitro. Oxytocin was used as a standard drug though it is a biochemically synthesized hormone that act on distant hormone receptor to induce contractions. The methanol extract was prepared using cold maceration method. Matured female non pregnant albino rats were used for this study using their uterine smooth muscle tissue. The extracts 667µg/ml, 1334µg/ml and 2668µg/ml was applied respectively to the organ bath containing the uterine tissue and using a digital physiological recorder, the extract was found to induce contraction of the uterine tissue. The extract produced its optimum frequency at 2668µg/ml (57.10%). The extract like oxytocin induce contraction by increasing Ca2+ influx which activates Ca2+ dependent cytosolic protein (calmodulin) to form Ca2+-calmodulin complex.

TABLE OF CONTENTS 

CHAPTER ONE 

Introduction    

CHAPTER TWO

LITERATURE REVIEW       

CHAPTER THREE

3.1 APPARATUS AND EQUIPMENTS

CHAPTER FOUR

RESULT AND ANALYSIS

CHAPTER FIVE 

DISCUSSION, CONCLUSION AND RECOMMENDATIONS